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semi dry iblot transfer system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher semi dry iblot transfer system
    Semi Dry Iblot Transfer System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iblot+semi+dry+transfer+system/Semi+dry+blot+transfer+buffer/pm40610719-263-29-33
    Average 97 stars, based on 1 article reviews
    semi dry iblot transfer system - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Membrane:

    Article Title: LNA antisense oligonucleotides for the modulation of Myc expression
    Article Snippet: .. Gels were transferred to blotting membrane with iBlot semi-dry transfer system (Invitrogen). ..

    Article Title: Dynamic plasticity within the EMT spectrum, rather than static mesenchymal traits, drives tumor heterogeneity and metastatic progression of breast cancers
    Article Snippet: For western blot, lysates were collected on-plate with 1x RIPA buffer (EMD Millipore #20-188) with protease and phosphatase inhibitors (ThermoScientific #1861280). .. Lysates were sonicated and cleared before quantification with a Bradford Protein assay (BioRad) and loaded at 50ug per lane and run on a NuPage Bis-Tris gel (ThermoFisher) and transferred to nitrocellulose membrane with the iBlot semi-dry transfer system (ThermoFisher) and blocked in 5% milk in TBST before staining with Fibronectin (BD #610078, 1:10,000), ZEB1 (LSBio ## 1:2000), E-cadherin (BD #610182, 1:1000), Vimentin (Cell Signaling Technology; CST #5741, 1:2000), RUNX1 (CST #4336, 1:2000), RUNX2 (CST #12556, 1:2000), RUNX3 (CST #9647, 1:2000), CBFb (Abcam ab33516, 1:2000), Snail (CST #3879, 1:1000), Twist1/2 (abcam ab50887, 1:50), and CoxIV (CST #11967, 1:2000) overnight in 5% milk in TBST. .. LiCor secondary antibodies, IRDye goat anti-rabbit and goat anti-mouse, 800CW (LiCor #925-32219) and 680RD (LiCor #925-68076), applied at 1:10,000 for 1 hour RT in 5% milk in TBST before imaging on the LiCor Oddessey CLx Digital Imager.

    Article Title: Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition
    Article Snippet: Lysates were subjected to FLAG immunoprecipitation using FLAG M2 agarose beads (Sigma Aldrich) for 2 h at 4 °C. .. Immunoprecipitates were washed three times in lysis buffer and resolved on 4–12% NuPAGE Bis-Tris precast gels (Invitrogen), transferred to PVDF membrane using the iBlot semi-dry transfer system (Invitrogen), blocked in either 1% non-fat dried milk or 2% BSA (Anti-Ubiquitin) in TBS-T (TBS, 0.1% Tween-20), and incubated with the relevant primary antibodies: Anti-FLAG HRP (A8592, Sigma Aldrich, 1:10,000 dilution), Anti-TRIM25 HRP (ab200788, AbCam, 1:5000 dilution), Anti-NS1 HRP (NS1-23–1) (sc130568, Santa-Cruz Biotechnology, 1:500 dilution), Anti-Ubiquitin HRP (PD41) (sc8017, Santa-Cruz Biotechnology, 1:500 dilution). ..

    Sonication:

    Article Title: Dynamic plasticity within the EMT spectrum, rather than static mesenchymal traits, drives tumor heterogeneity and metastatic progression of breast cancers
    Article Snippet: For western blot, lysates were collected on-plate with 1x RIPA buffer (EMD Millipore #20-188) with protease and phosphatase inhibitors (ThermoScientific #1861280). .. Lysates were sonicated and cleared before quantification with a Bradford Protein assay (BioRad) and loaded at 50ug per lane and run on a NuPage Bis-Tris gel (ThermoFisher) and transferred to nitrocellulose membrane with the iBlot semi-dry transfer system (ThermoFisher) and blocked in 5% milk in TBST before staining with Fibronectin (BD #610078, 1:10,000), ZEB1 (LSBio ## 1:2000), E-cadherin (BD #610182, 1:1000), Vimentin (Cell Signaling Technology; CST #5741, 1:2000), RUNX1 (CST #4336, 1:2000), RUNX2 (CST #12556, 1:2000), RUNX3 (CST #9647, 1:2000), CBFb (Abcam ab33516, 1:2000), Snail (CST #3879, 1:1000), Twist1/2 (abcam ab50887, 1:50), and CoxIV (CST #11967, 1:2000) overnight in 5% milk in TBST. .. LiCor secondary antibodies, IRDye goat anti-rabbit and goat anti-mouse, 800CW (LiCor #925-32219) and 680RD (LiCor #925-68076), applied at 1:10,000 for 1 hour RT in 5% milk in TBST before imaging on the LiCor Oddessey CLx Digital Imager.

    Bradford Protein Assay:

    Article Title: Dynamic plasticity within the EMT spectrum, rather than static mesenchymal traits, drives tumor heterogeneity and metastatic progression of breast cancers
    Article Snippet: For western blot, lysates were collected on-plate with 1x RIPA buffer (EMD Millipore #20-188) with protease and phosphatase inhibitors (ThermoScientific #1861280). .. Lysates were sonicated and cleared before quantification with a Bradford Protein assay (BioRad) and loaded at 50ug per lane and run on a NuPage Bis-Tris gel (ThermoFisher) and transferred to nitrocellulose membrane with the iBlot semi-dry transfer system (ThermoFisher) and blocked in 5% milk in TBST before staining with Fibronectin (BD #610078, 1:10,000), ZEB1 (LSBio ## 1:2000), E-cadherin (BD #610182, 1:1000), Vimentin (Cell Signaling Technology; CST #5741, 1:2000), RUNX1 (CST #4336, 1:2000), RUNX2 (CST #12556, 1:2000), RUNX3 (CST #9647, 1:2000), CBFb (Abcam ab33516, 1:2000), Snail (CST #3879, 1:1000), Twist1/2 (abcam ab50887, 1:50), and CoxIV (CST #11967, 1:2000) overnight in 5% milk in TBST. .. LiCor secondary antibodies, IRDye goat anti-rabbit and goat anti-mouse, 800CW (LiCor #925-32219) and 680RD (LiCor #925-68076), applied at 1:10,000 for 1 hour RT in 5% milk in TBST before imaging on the LiCor Oddessey CLx Digital Imager.

    Staining:

    Article Title: Dynamic plasticity within the EMT spectrum, rather than static mesenchymal traits, drives tumor heterogeneity and metastatic progression of breast cancers
    Article Snippet: For western blot, lysates were collected on-plate with 1x RIPA buffer (EMD Millipore #20-188) with protease and phosphatase inhibitors (ThermoScientific #1861280). .. Lysates were sonicated and cleared before quantification with a Bradford Protein assay (BioRad) and loaded at 50ug per lane and run on a NuPage Bis-Tris gel (ThermoFisher) and transferred to nitrocellulose membrane with the iBlot semi-dry transfer system (ThermoFisher) and blocked in 5% milk in TBST before staining with Fibronectin (BD #610078, 1:10,000), ZEB1 (LSBio ## 1:2000), E-cadherin (BD #610182, 1:1000), Vimentin (Cell Signaling Technology; CST #5741, 1:2000), RUNX1 (CST #4336, 1:2000), RUNX2 (CST #12556, 1:2000), RUNX3 (CST #9647, 1:2000), CBFb (Abcam ab33516, 1:2000), Snail (CST #3879, 1:1000), Twist1/2 (abcam ab50887, 1:50), and CoxIV (CST #11967, 1:2000) overnight in 5% milk in TBST. .. LiCor secondary antibodies, IRDye goat anti-rabbit and goat anti-mouse, 800CW (LiCor #925-32219) and 680RD (LiCor #925-68076), applied at 1:10,000 for 1 hour RT in 5% milk in TBST before imaging on the LiCor Oddessey CLx Digital Imager.

    Western Blot:

    Article Title: Liquid and Hydrogel Phases of PrP C Linked to Conformation Shifts and Triggered by Alzheimer’s Amyloid-β Oligomers
    Article Snippet: .. For immunoblotting of human brain lysates and PrP C -induced condensates of brain material the gels were transferred onto nitrocellulose membranes (ThermoFisher, IB301001) using iBlot semi-dry transfer system (ThermoFisher). ..

    Incubation:

    Article Title: Live-attenuated RNA hybrid vaccine technology provides single-dose protection against Chikungunya virus.
    Article Snippet: .. VLP solutions were reduced with NuPage 10 reducing agent and NuPAGE LDS sample buffer (Invitrogen) and denatured by incubation at 95 C for 10 min before loading on duplicate Novex Wedgewell 4%–20% gradient precast PAGE gels and being run at 120 V in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) SDS running buffer for 1 h. The gels were then transferred to polyvinylidene fluoride (PVDF) membranes using the Invitrogen iBlot semi-dry transfer system with a 6-min transfer step. ..

    Article Title: Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition
    Article Snippet: Lysates were subjected to FLAG immunoprecipitation using FLAG M2 agarose beads (Sigma Aldrich) for 2 h at 4 °C. .. Immunoprecipitates were washed three times in lysis buffer and resolved on 4–12% NuPAGE Bis-Tris precast gels (Invitrogen), transferred to PVDF membrane using the iBlot semi-dry transfer system (Invitrogen), blocked in either 1% non-fat dried milk or 2% BSA (Anti-Ubiquitin) in TBS-T (TBS, 0.1% Tween-20), and incubated with the relevant primary antibodies: Anti-FLAG HRP (A8592, Sigma Aldrich, 1:10,000 dilution), Anti-TRIM25 HRP (ab200788, AbCam, 1:5000 dilution), Anti-NS1 HRP (NS1-23–1) (sc130568, Santa-Cruz Biotechnology, 1:500 dilution), Anti-Ubiquitin HRP (PD41) (sc8017, Santa-Cruz Biotechnology, 1:500 dilution). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Live-attenuated RNA hybrid vaccine technology provides single-dose protection against Chikungunya virus.
    Article Snippet: .. VLP solutions were reduced with NuPage 10 reducing agent and NuPAGE LDS sample buffer (Invitrogen) and denatured by incubation at 95 C for 10 min before loading on duplicate Novex Wedgewell 4%–20% gradient precast PAGE gels and being run at 120 V in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) SDS running buffer for 1 h. The gels were then transferred to polyvinylidene fluoride (PVDF) membranes using the Invitrogen iBlot semi-dry transfer system with a 6-min transfer step. ..

    Lysis:

    Article Title: Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition
    Article Snippet: Lysates were subjected to FLAG immunoprecipitation using FLAG M2 agarose beads (Sigma Aldrich) for 2 h at 4 °C. .. Immunoprecipitates were washed three times in lysis buffer and resolved on 4–12% NuPAGE Bis-Tris precast gels (Invitrogen), transferred to PVDF membrane using the iBlot semi-dry transfer system (Invitrogen), blocked in either 1% non-fat dried milk or 2% BSA (Anti-Ubiquitin) in TBS-T (TBS, 0.1% Tween-20), and incubated with the relevant primary antibodies: Anti-FLAG HRP (A8592, Sigma Aldrich, 1:10,000 dilution), Anti-TRIM25 HRP (ab200788, AbCam, 1:5000 dilution), Anti-NS1 HRP (NS1-23–1) (sc130568, Santa-Cruz Biotechnology, 1:500 dilution), Anti-Ubiquitin HRP (PD41) (sc8017, Santa-Cruz Biotechnology, 1:500 dilution). ..



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